Composite

Part:BBa_K3206009:Design

Designed by: Matthew Rogan   Group: iGEM19_Newcastle   (2019-10-15)


GcvR under constitutive expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part is made up of a constitutively expressing promoter (BBa_J23100), RBS (BBa_B0034), GcvR CDS (BBa_K3206005) and a terminator (BBa_B0015). The composite part was designed in benching and made to be compatible with Type IIs assembly, with BsaI restriction sites and fusion sites compatible with a DVK-AF level-1 pOdd destination vector. The part was ordered as a gBlock from IDT.

Source

This composite part was made of basic parts sourced from the iGEM Registry of Standard Biological Parts.

References

Ghrist, Angela C. & Stauffer, George V, 1998. Promoter Characterization and Constitutive Expression of the Escherichia coli gcvR Gene. The Journal of Bacteriology, 180(7), pp.1803–1807.